Volume No. :   10

Issue No. :  2

Year :  2018

ISSN Print :  0975-4407

ISSN Online :  2321-5836


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Comparison of the antimicrobial activity of the phyto-constituents obtained from the stem bark and leaf extracts of Phyllanthus emblica L. against different strains of Staphylococcus aureus and Salmonella typhi.



Address:   Serene Adak*, Dipesh Chakraborty, Himangshu S. Maji*, Satakshi Basu, Pradip Roy, Sutanuka Mitra, Nabanita Mukherjee, Samarendra Barik, Arunava Goswami
Bengal School of Technology & Management, Sugandha, Delhi Road, Chinsurah, West Bengal 712102 and Indian Statistical Institute , Plot No. 203, Trunk Road, Bonhooghly Government Colony, Barrackpore, Kolkata, West Bengal 700108
*Corresponding Author
DOI No: 10.5958/2321-5836.2018.00009.5

ABSTRACT:
Phyllanthus (family Phyllanthaceae) is highly important medicinal plant which has been used traditionally for varieties of medicinal purposes of both microbial and non-microbial ailments. An in-vitro investigation is undertaken against five different strains of gram positive pathogen Staphylococcus aureus and Gram negative pathogen Salmonella typhi with the extracts from leaf and stem berk of Phyllanthus emblica L. Plant. Phytochemical extracts were prepared in ethanol by maceration method and screened by chemical reactions of standard procedures. The flavonoid contents were further screened by TLC method. Ethanol extract of leaf and bark were characterised by FTIR as a supportive determination for the constituent. The extracts were applied on five strains of S. aureus e-145, ML-366, ML-15, ML-276, ML-145 and S typhi .A-2467, e-3404, E-145, B-111 and NCTC-14 with different doses of leaf and berk extracts. The susceptibility testing and the Minimal inhibitory concentration (MIC) determination showed 250 mg/ml and 25 mg/ml of leaf and bark extract respectively are effective on most of the strains of S. aureus, on the other hand 100 mg/ml and 50 mg/ml of leaf and bark extract respectively were successful to inhibit growth of the strains of Gram negative S. typhi. Phytochemical derivation revealed presence of aglycones and steroid glycosides as major components, orthosiphonis folium and petasins folium. Our experiments focus the scientific parameter for the traditional therapeutic use of other parts e.g. leaf and bark rather than only fruit of Phyllanthus emblica L. and enlighten the exploration for the further pharmaceutical use.
KEYWORDS:
Phyllanthusemblica, Flavonoids, Antimicrobial activity, S. typhi, S.aureus.
Cite:
Serene Adak, Dipesh Chakraborty, Himangshu S. Maji, Satakshi Basu, Pradip Roy, Sutanuka Mitra, Nabanita Mukherjee, Samarendra Barik, Arunava Goswami. Comparison of the antimicrobial activity of the phyto-constituents obtained from the stem bark and leaf extracts of Phyllanthus emblica L. against different strains of Staphylococcus aureus and Salmonella typhi. Res. J. Pharmacology and Pharmacodynamics.2018; 10(2): 53-60.
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