Kamalakshi Krishnamurthy1, Thant Zin2, K. Priyamvatha3, Mahadeva Rao U.S.2, Suganya M.4
1Additional Director & Director (i/c)-Retired, Forensic Sciences Department, Mylapore, Chennai, India.
2School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin (UniSZA), Kuala Terengganu, Malaysia.
3Former Student of Women's Christian College, Chennai, India.
4People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India.
*Corresponding Author E-mail: raousm@gmail.com, raousm@unisza.edu.my
ABSTRACT:
Nerium Odorum is an important medicinal plant in India. It is a cardiac glycoside. All parts of the plant are poisonous. Recent studies have thrown light into the fact that it has pharmacological, antibacterial, antifungal, anti-inflammatory, antipyretic, anti-asthmatic and anticancer activities. This study envisages a new developing as well as identification method for Pink/white oleander by Thin Layer Chromatography.
KEYWORDS: Nerium Odorum, Cardiac glycoside, Anti-inflammatory, Anti-asthmatic.
INTRODUCTION:
Plant poisoning is the most common type of poisoning in India due its easy availability. The white and pink flowers of Nerium Odorum are being offered to Hindu Gods for worshipping. It is also an important medicinal plant used in folk medicine. Nerium Odorum is a cardiac glycoside acting directly on the heart. It is an ornamental shrub widely grown all over India. It bears flowers in terminal clusters. The flowers are white or pink in colour. Leaves are narrow, lanceolate and dark green in colour. Seeds are long, narrow and cylindrical about 5 to 10 cm long.
All parts of the plant are poisonous, particularly leaves, root, stem and seeds. The active Principles are Nerine and Oleandrin. Roots and leaves are often used as a paste or decoction for suicidal purposes. Homicide is rare. It is also used as abortificient (abortifacient) and cattle poison.
Recent research has thrown light into the fact that it has pharmacological, antibacterial, antifungal, anti-inflammatory, antipyretic, anti-asthmatic and anticancer activities.
METHODS:
This paper envisages a new developing as well as identification method for Pink/white oleander by Thin Layer Chromatography.
Thin Layer Chromatography is used due to its low cost, simplicity, quick development time, high sensitivity and good reproducibility.
TLC is a good alternative method to some of the reported, expensive instrumental methods.
Silica Gel GF 254 Plates used.
System:
Dichloromethane: Cyclohexane: Methanol
40 9 1.7
System allowed to saturate. Control Oleander is spotted on the TLC plate. Plate is allowed to run to sufficient height, removed and dried. Plate is sprayed with saturated alcoholic solution of meta-dinitrobenzene followed by strong alcoholic Potassium Hydroxide. Spot revealed at a Rf 0.6.
REFERENCES:
1. Indian Medicinal Plants - By Kirti and Basuthkar
2. Textbook Medical Jurisprudence and Toxicology - By Jaising Modi
3. Forensic Medicine and Toxicology - By V. Dekal.
4. Parikh's Textbook of Medica Jurisprudence & Toxicology - By C.K. Parikh.
5. Essentials of Forensic Medicine & Toxicology - By K.S. Narayan Reddy & O.P. Murthy.
6. Thin Layer Chromatography - By Egon Stahl.
Received on 08.06.2022 Modified on 29.06.2022
Accepted on 21.07.2022 ŠA&V Publications All right reserved
Res. J. Pharmacology and Pharmacodynamics.2022;14(3):183-184.
DOI: 10.52711/2321-5836.2022.00032