VN Shah, DB Doshi, MB Shah, PA Bhatt.
V.N. Shah1*, D.B. Doshi1, M.B. Shah2 and P.A. Bhatt2
1APMC College of Pharmaceutical Education and Research, Himatnagar-380001, Gujarat, India
2L.M. College of Pharmacy, Ahmedabad -380009, Gujarat, India
Volume - 2,
Issue - 4,
Year - 2010
Objective: Punarnavashtak (PN) kwath a classical Ayurvedic formulation mentioned in Ayurvedic literature “Bhaishyajyaratnavali” for hepatic disorders and asthma. The present study investigated the hepatoprotective activity of PN kwath to validate the traditional use of this formulation.
Materials and methods: PN kwath was prepared in the laboratory according to the method given in Ayurvedic literature. Preliminary phytochemical screening was performed to determine the presence of phytoconstituents. Hepatoprotective and antioxidant activity was evaluated against paracetamol induced hepatotoxicity in rats
Results: Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, saponins and bitter principal in PN kwath. Administration of PN kwath produced significant hepatoprotective effect as demonstrated by decrease level of serum liver marker enzymes like AST, ALT, ALP, SBRN and increase protein level. It also showed antioxidant activity by increase in activity of GSH, SOD, CAT and decrease in TBARS level compare to paracetamol treated group. A comparative histopathological study of liver exhibited almost normal architecture, as compared to paracetamol treated group.
Discussion and Conclusion: It can be concluded that PN kwath protects hepatocyte from paracetamol-induced liver damages due to its antioxidant effect on hepatocytes
Cite this article:
VN Shah, DB Doshi, MB Shah , PA Bhatt. Evaluation of Hepatoprotective and Antioxidant Activity of Classical Ayurvedic Formulation Punarnavashtak Kwath Against Paracetamol Induced Hepatotoxicity in Rats. Research J. Pharmacology and Pharmacodynamics. 2010; 2(4): 283-288.