Author(s):
Kiran Bala, Ajeet Pal Singh, Indu Melkani, Amar Pal Singh
Email(s):
kiranbala814626610@gmail.com , ajeetakarpuria@gmail.com , ikshitamelkani@gmail.com , amar.psingh75@gmail.com
DOI:
10.52711/2321-5836.2025.00026 
Address:
Kiran Bala1, Ajeet Pal Singh1, Indu Melkani2, Amar Pal Singh3
1St. Soldier Institute of Pharmacy, Jalandhar Near Amritsar Road, Behind NIT, Jalandhar, Punjab.
2Assistant Professor, Amity Institute of Pharmacy, Amity University, Lucknow, U.P., India.
3Lovely Professional University, Phagwara, Kapurthala, Punjab, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 3,
Year - 2025
ABSTRACT:
Background: Inflammatory bowel disease (IBD) is an idiopathic chronic inflammatory gastrointestinal disease that includes Crohn's disease and ulcerative colitis. Symptoms include diarrehoea, stomach discomfort, blood in the stool and other similar issues. Since there are no safe and effective treatments for gut inflammation, new treatments developed to efficiently treat the disease's symptoms and implications. Methods: The development of collitus was promoted by the injection of 3% acetic acid into the rat colon through the rectum. Macroscopic, biochemical, histological and disease activity scoring of the colon were performed after 5 days of infusion to evaluate colonic damage. A study on the activity of Holarrhena antidysenterica and coriander sativum Linn was investigated in collitus-induced 36 albino rats randomized into five groups (n=6). Control Group I given the normal saline, Group II give the Acetic acid 3% through rectum, Group III give the Budesonide (0.5mg/kg) through oral. Group IV give the aqueous extract of Holarrhena antidysenterica (HA) (40mg/kg) through oral, Group V give the aqueous extract of Coriander sativum Linn. (CS) (40mg/kg) through oral and in Group VI given the combination of both extract HA and CS. Both treatment drugs given 2 days before induction of collitus. Results: A significant colon inflammatory response to acetic acid infusion is observed macroscopically and histopathologically. Additionally, it results in a decrease in GSH, SOD and an increase in MPO level. Thus in current study aq. extracts of Holarrhena antidysenterica and Coriandrum sativum were administered for 7 days i.e. commencing 2 days prior to the induction of acetic acid and continuing for 5 days after. Compared to disease control there was a significantly reduced amount of macroscopic damage and disease activity score. Additionally, it increased intestinal levels of reduced GSH, SOD and significantly decreased MPO levels. Conclusion: Inflammation is inhibited by coriander sativum while the primary symptoms of IBD are inhibited by Holarrhena antidysenterica. Inflammatory bowel disease can be treated more effectively with a combination of these two medications which also eliminates the side effects of glucocorticoids (Budesonide).
Cite this article:
Kiran Bala, Ajeet Pal Singh, Indu Melkani, Amar Pal Singh. Exploring Anti-inflammatory effect of Holarrhena antidysenterica and Coriandrum sativum Linn in the Digestive System disease IBD in Rats. Research Journal of Pharmacology and Pharmacodynamics. 2025;17(3):159-164. doi: 10.52711/2321-5836.2025.00026
Cite(Electronic):
Kiran Bala, Ajeet Pal Singh, Indu Melkani, Amar Pal Singh. Exploring Anti-inflammatory effect of Holarrhena antidysenterica and Coriandrum sativum Linn in the Digestive System disease IBD in Rats. Research Journal of Pharmacology and Pharmacodynamics. 2025;17(3):159-164. doi: 10.52711/2321-5836.2025.00026 Available on: https://rjppd.org/AbstractView.aspx?PID=2025-17-3-1
REFERENCES:
1. Sadraei H, Asghari G, Khanabadi M, Minaiyan M. Anti-inflammatory effect of apigenin and hydroalcoholic extract of Dracocephalum kotschyi on acetic acid-induced colitis in rats. Res Pharm Sci. 2017; 12(4): 322–9.
2. Abdalla MI, Herfarth H. HHS Public Access. 2017; 17(11): 1549–59.
3. Rahimi R, Shams-ardekani MR, Abdollahi M. A review of the efficacy of traditional Iranian medicine for inflammatory bowel disease. 2010; 16(36): 4504–14.
4. Fakhoury M, Negrulj R, Mooranian A, Al-Salami H. Inflammatory bowel disease: Clinical aspects and treatments. J Inflamm Res. 2014; 7(1): 113–20.
5. Martinon F, Tschopp J. NLRs join TLRs as innate sensors of pathogens. Trends Immunol. 2005; 26(8): 447–54.
6. Hugot JP, Chamaillard M, Zouali H, Lesage S, Cézard JP, Belaiche J, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn’s disease. Nature. 2001; 411(6837): 599–603.
7. Sevrin G, Massier S, Chassaing B, Agus A, Delmas J, Denizot J, et al. Adaptation of adherent-invasive E. coli to gut environment: Impact on flagellum expression and bacterial colonization ability. Gut Microbes [Internet]. 2020; 11(3): 364–80. Available from: https://doi.org/10.1080/19490976.2017.1421886
8. Gawron LM, Goldberger A, Gawron AJ, Hammond C, Keefer L. The impact of hormonal contraception on disease-related cyclical symptoms in women with inflammatory bowel diseases. Inflamm Bowel Dis. 2014; 20(10): 1729–33.
9. Yu YR, Rodriguez JR. Seminars in Pediatric Surgery Clinical presentation of Crohn’s, ulcerative colitis, and indeterminate colitis: Symptoms, extraintestinal manifestations, and disease phenotypes. Semin Pediatr Surg [Internet]. 2017; 26(6): 349–55. Available from: http://dx.doi.org/10.1053/ j.sempedsurg.2017.10.003
10. Walia D, Kaur G, Jaggi AS, Bali A. Exploring the therapeutic potential of sodium benzoate in acetic acid-induced ulcerative colitis in rats. J Basic Clin Physiol Pharmacol. 2019; 1–9.
11. Cagin YF, Parlakpinar H, Vardi N, Polat A, Atayan Y, Erdogan MA, et al. Effects of dexpanthenol on acetic acid-induced colitis in rats. Exp Ther Med. 2016; 12(5): 2958–64.
12. Kumar J, Sakthivel KM, Guruvayoorappan C, M BG V. ScienceDirect Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of in fl ammatory mediators and cytokines. Biomed Pharmacother [Internet]. 2023; 91(2017): 1113–21. Available from: http://dx.doi.org/10.1016/ j.biopha.2017.05.057
13. Silva I, Pinto R, Mateus V. Preclinical study in vivo for new pharmacological approaches in inflammatory bowel disease: a systematic review of chronic model of TNBS-induced colitis. J Clin Med. 2019; 8(10): 1574.
14. Klebanoff SJ. Myeloperoxidase. Proc Assoc Am Physicians. 1999; 111(5): 383–9.
15. Sinha S. Scientific. 2018: 1–10.
16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem [Internet]. 1951; 193(1): 265–75. Available from: http://dx.doi.org/ 10.1016/S0021-9258(19)52451-6
17. Bilski J, Mazur-Bialy A, Wojcik D, Magierowski M, Surmiak M, Kwiecien S, et al. Effect of forced physical activity on the severity of experimental colitis in normal weight and obese mice. Involvement of oxidative stress and proinflammatory biomarkers. Nutrients. 2019; 11(5).
18. Khan A, Khan SR, Gilani AH. Studies on the in vitro and in vivo antiurolithic activity of Holarrhena antidysenterica. Urol Res. 2012; 40(6): 671–81.
19. Heidari B, Sajjadi SE, Minaiyan M. Effect of Coriandrum sativum hydroalcoholic extract and its essential oil on acetic acid- induced acute colitis in rats. Avicenna J Phytomedicine [Internet]. 2016; 6(2): 205–14. Available from: http://www.ncbi.nlm.nih.gov/ pubmed/27222834%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4877963