Author(s):
Sudipta Rani Bera, Suman Pattanayak, Lakshmi Kanta Kanthal, Ghalib Iqubal, Shyamal Manna, Sk Sayan Gazal, Souvik Hanra
Email(s):
berasudipta693@gmail.com
DOI:
10.52711/2321-5836.2023.00009
Address:
Sudipta Rani Bera*, Suman Pattanayak, Lakshmi Kanta Kanthal, Ghalib Iqubal, Shyamal Manna, Sk Sayan Gazal, Souvik Hanra
Haldia Institute of Pharmacy, Haldia, Purba Medinipur, West Bengal- 721657.
*Corresponding Author
Published In:
Volume - 15,
Issue - 2,
Year - 2023
ABSTRACT:
Erigeron bonariensis commonly known as Gulava has been used for thousands of year in the ayurveda for its various chemical constituent. This study was conducted to evaluate the locomotor activity of Erigeron bonariensis on zebrafish models as the locomotor behaviour of zebrafish has been widely used to study neuro-behaviour. The plant was collected and authenticated by botanical survey of India, Kolkata. The aerial parts of E. bonariensis were extracted using of methanol by maceration method and phytochemical tests were performed as per standard procedure. Then fishes were divided into 4 Groups of 6 animals each (n=6). Group 1 – served as control, Group 2 Group 3 and Group 4 – were treated with test substance at three dose (1 mg/ml, 2 mg/ml, 4 mg/ml) levels. Thenwe observed locomotor activity including total distance travelled, speed to evaluate the impact of E. bonariensis on the adult Zebra fish. The study showed the presence of glycosides, flavonoids, Amino acid, tannins and absence of Alkaloids, carbohydrates and saponin. The zebrafish was treated with methanolic extract of E. bonariensis which showed increase in locomotor activity. The extract did not show any kind of toxic effect during and after the observation period. Results in this study showed that CNS stimulating activity was found in E. bonariensis.
Cite this article:
Sudipta Rani Bera, Suman Pattanayak, Lakshmi Kanta Kanthal, Ghalib Iqubal, Shyamal Manna, Sk Sayan Gazal, Souvik Hanra. Locomotor activity on zebra fish model using methanolic extract of Erigeron bonariensis L. Research Journal of Pharmacology and Pharmacodynamics. 2023;15(2):45-8. doi: 10.52711/2321-5836.2023.00009
Cite(Electronic):
Sudipta Rani Bera, Suman Pattanayak, Lakshmi Kanta Kanthal, Ghalib Iqubal, Shyamal Manna, Sk Sayan Gazal, Souvik Hanra. Locomotor activity on zebra fish model using methanolic extract of Erigeron bonariensis L. Research Journal of Pharmacology and Pharmacodynamics. 2023;15(2):45-8. doi: 10.52711/2321-5836.2023.00009 Available on: https://rjppd.org/AbstractView.aspx?PID=2023-15-2-1
REFERENCES:
1. Allen DG, Lamb GD, Westerblad H. Skeletal muscle fatigue: cellular mechanisms. Physiol Rev. 2008 Jan;88(1):287-332. doi: 10.1152/physrev.00015.2007. PMID: 18195089.
2. Chavan G. M., Vasaikar Rupali S., Patil J. K.et al. Study of CNS Depressant activity of Ethanolic extract of Madhuca longifolia Flower. Res. J. Pharmacology & Pharmacodynamics.2019; 11(4):127-131.
3. Nidhi J. Kapadiya, Dhrubo Jyoti Sen. Structure Activity Relationship Studies of Synthesized Diamides on CNS Depression and Sleeping Time Potentiation Effect. Asian J. Research Chem. 3(4): Oct. - Dec. 2010; Page 1027-1029.
4. Arunachalam M, Raja M, Vijayakumar C et al. Natural history of zebrafish (Danio rerio) in India. Zebrafish. 2013 Mar;10(1):1-14. doi: 10.1089/zeb.2012.0803. PMID: 23590398.
5. Bagnall MW, Schoppik D. Development of vestibular behaviors in zebrafish. Curr Opin Neurobiol. 2018 Dec;53:83-89. doi: 10.1016/j.conb.2018.06.004. Epub 2018 Jun 26. PMID: 29957408; PMCID: PMC6242715.
6. Egan RJ, Bergner CL, Hart PC et al. Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behav Brain Res. 2009 Dec 14;205(1):38-44. doi: 10.1016/j.bbr.2009.06.022. Epub 2009 Jun 18. PMID: 19540270; PMCID: PMC2922906.
7. Jijith U. S., Sudhakaran Nair C. R., Pramod K. Effect of Alcoholic Extract of Stem Bark of Anamirta cocculus Willd on Central Nervous System. Res. J. Pharmacognosy and Phytochem. 2016; 8(3): 141-144.
8. Wang, A.; Wu, H.; Zhu, X et al. Species identification of Conyza bonariensis assisted by chloroplast genome sequencing. Front.Genet. 2018, 9, 374.https://doi.org/10.3389/fgene.2018.00374
9. Sabarinath C, Nandhu T, Sudhakar P et al, Shanmuganath C. Teratogenic effect of Ethanolic extract of Solanum xanthocarpum berries in Zebrafish embryo. Research J. Pharm. and Tech. 2020; 13(11):5313-5316
10. Kumar A. K., Ramachandra S. S., Narsu L. Pharmacognostic and phytochemical investigations of roots of Hibiscus micranthus Linn. Research Journal of pharmaceutical, Biological and Chemical Sciences (RJPBCS) 2010; 1(4): 324–337. DOI:10.1016/S0975-3575(10)80074-4
11. Nguyen H. C., Lin K.-H., Hang M.-Y et al. (2018). Antioxidant Activities of the Methanol Extracts of Various Parts of Phalaenopsis Orchids with White, Yellow, and Purple Flowers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 457–465. https://doi.org/10.15835/nbha46211038
12. Krishnaiah D, Devi T, Bono A. et al. Studies on phytochemical constituents of six Malaysian medicinal plants. Journal of Medicinal Plants Research. 2009;3(2):67–72. https://doi.org/10.5897/JMPR.9001153
13. Shivananda Nayak B, Sivachandra Raju S, Orette FA et al. Effects of Hibiscus rosa sinensis L (Malvaceae) on wound healing activity: a preclinical study in a Sprague Dawley rat. Int J Low Extrem Wounds. 2007 Jun;6(2):76-81. doi: 10.1177/1534734607302840. PMID: 17558005.
14. Mekonnen A, Sidamo T, Asres K et al. In vivo wound healing activity and phytochemical screening of the crude extract and various fractions of Kalanchoe petitiana A. Rich (Crassulaceae) leaves in mice. J Ethnopharmacol. 2013 Jan 30;145(2):638-46. doi: 10.1016/j.jep.2012.12.002. Epub 2012 Dec 8. PMID: 23228912.
15. Jaganathan Murugasan Kuppuswamy, Barathi Seetharaman. Mancozeb exposure at sublethal concentration alters the transcription of the genes related to apoptosis in the adult zebrafish (Danio rerio) brain. Research J. Pharm. and Tech. 2020; 13(10):4801-4804.
16. Siva Athitya L. V., Muthu Vijai Bharath V., Prakash P. Screening of Gracilaria corticata Extracts for Acetyl cholin esterase Inhibitory Activity. Research J. Pharm. and Tech 2018; 11(9): 3848-3850.
17. Kimmel CB, Ballard WW, Kimmel SR et al. Stages of embryonic development of the zebrafish. Dev Dyn. 1995 Jul;203(3):253-310. doi: 10.1002/aja.1002030302. PMID: 8589427.
18. Sudha N, Arambakkam Janardhanam Vanisree. Neuroprotective role of Curcuma amada in F1 progeny of Juvenile Danio rerio (Zebrafish) from Nickel Chloride Induced Maternal Stress in Gestationally Challenged Adult Fishes. Research Journal of Pharmacy and Technology. 2021; 14(8):4215-0.
19. Purnomo Y., Aini N., Noerhayati E. Acute Toxicity Level of Pulutan (Urena lobata) Leaf Extract on Zebrafish (Danio rerio) and its Analysis by In Silico Study. Research Journal of Pharmacy and Technology. 2022; 15(6):2477-2.
20. Spence R, Gerlach G, Lawrence C el al. The behaviour and ecology of the zebrafish, Danio rerio. Biol Rev Camb Philos Soc. 2008 Feb;83(1):13-34. doi: 10.1111/j.1469-185X.2007.00030.x. Epub 2007 Dec 17. PMID: 18093234.
21. Manisha S., Sanjay C., Ritu G. Evaluation of Central Nervous System Depressant Action of Catharanthus roseus G. Don Leaves in Mice. Research J. Pharm. and Tech 2021; 14(3):1719-1722.
22. Ashirbad N., Sai Aparna, Rudra Narayan S. et al. Effect of Amlodipine in Proliferative Retinopathy: Biochemical Estimation and Zebrafish (Danio rerio) Modeling. Research Journal of Pharmacy and Technology. 2022; 15(3):1114-8.