Author(s):
Junaid S Shaikh, Sunil Jabra Tadvi, Shashikant D Barhate
Email(s):
skjunaid.pharm@gmail.com
DOI:
10.52711/2321-5836.2024.00047 
Address:
Junaid S Shaikh, Sunil Jabra Tadvi, Shashikant D Barhate
Shree Sureshdada Jain Institute of Pharmaceutical Education and Research, Jamner, Jalgaon, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 16,
Issue - 4,
Year - 2024
ABSTRACT:
This research article is based on the antibacterial activity of neem. We compared the antibacterial activity of a standard antibiotic with our neem alcoholic extract using the cup-plate method. For this study, we utilized non-pathogenic bacteria, specifically Lactobacillus grown in MRS agar media prepared with tomato juice and meat extract. This bacterial culture was employed to evaluate the antibacterial activity of neem leaf extract. The entire study was conducted in a college laboratory to understand the concepts of microbial assay and the format of research paper writing. The benefit of using neem extract lies in its easy availability and low cost. However, neem is not used commercially due to its bitter taste and the challenging extraction process.
Cite this article:
Junaid S Shaikh, Sunil Jabra Tadvi, Shashikant D Barhate. Comparative Study of Antibacterial activity of neem leaf extract against standard benzyl penicillin by using In vitro cup plate method. Research Journal of Pharmacology and Pharmacodynamics. 2024;16(4):274-6. doi: 10.52711/2321-5836.2024.00047
Cite(Electronic):
Junaid S Shaikh, Sunil Jabra Tadvi, Shashikant D Barhate. Comparative Study of Antibacterial activity of neem leaf extract against standard benzyl penicillin by using In vitro cup plate method. Research Journal of Pharmacology and Pharmacodynamics. 2024;16(4):274-6. doi: 10.52711/2321-5836.2024.00047 Available on: https://rjppd.org/AbstractView.aspx?PID=2024-16-4-3
REFERENCES:
1. Dr. Chandrakant Kokare. Pharmaceutical Microbiology Principle and Application. Fourth Edition. Nirali Prakashan. 2007, 19.1
2. C.K. Kokate, A.P. Purohit, S.B. Ghokhale. Pharmacognosy. 56-Edition. Nirali Prakashan, Pune. 2019 Page No.19.3, 19.4
3. Ahmed N, Grainge M. Some promising plants for pest control under small scale forming operation in developing countries working paper resource system institute, East-West Centre, Honolulu, Hawai, USA. 1982, 55.
4. Shivpuri Asha, Sharma OP, Jhamaira SL. Fungitoxic properties of plant extracts against pathogenic fungi. J Mycol. Pl. Pathol. 1997; 27: 29-31
5. Abd Alla MS, Atalla KM, Elp-Sawi MAM. Effect of some plant waste extracts on growth and aflatoxin production by Aspergillus flavus, Annals Agric Sci., Ain Shams Univ. Cairo. 2001; 46: 579-592.
6. Wilson C, Solar JM, Ghaouth-El A, Wisniewski ME. Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Pl. Dis. 1997; 81: 201-210.
7. Grane M, Ahmad S. Handbook of Plants with Pest Control Properties. John Wiley and Sons, New York, 1988.
8. Chandrakant Kokare. Pharmaceutical Microbiology Experiments and Techniques. Fourth Edition, Career Publication. 2013; 222
9. Bansal RK, Gupta RK. Evaluation of plant extracts against Fusarium oxysporum, wilt pathogen of fenugreek. Indian Phytopath. 2000; 53(1): 107-108.
10. Dwivedi BP, Shukla BN. Effect of leaf extracts of some medicinal on spore germination of some Fusarium species. Karnataka J Agric. Sci. 2000; 13(1):153-154
11. Rajput SS. Management of smut in pearl millet. M.Sc. (Ag.) Thesis (JNKVV), College of Agriculture, Gwalior, 2000.
12. Dubey AK. Studies on leaf spot and flower blight of marigold M.Sc. (Ag) Thesis. JNKVV, Jabalpur (M.P.), 2001, 61.
13. Sharma RN. Studies on Alternaria blight of Guar (Cyamopsis tetragonoloba (L.) Taub.). M.Sc. (Ag) Thesis. JNKW, Jabalpur, 2004.
14. Alkhail. In-vitro evaluation of different plant extracts against some fungal pathogen. Pak. J Bol. 2005; 39(7): 2619-2624.