ABSTRACT:
An attempt has been made to assess the immunomodulatory activity of methanol extracts of whole plant of E. alba (1.6% wedelolactone) and C. asiatica (0.18% of asiaticoside) at five dose levels (dose-response relationship) ranging from 100 to 500mg/kg body wt. using carbon clearance, antibodytiter and cyclophosphamide immunosuppression parameters. In the case of E. alba, the phagocytic index and antibody titer increased significantly and the F ratios of the phagocytic indexand WBC count were also significant. Regression analysis showed linearity in patterns of the dose response relationship, greatest in the case of the phagocytic index, moderate in the WBC count andlowest in the antibody titer. For C. asiatica, significant increases in the phagocytic index and total WBC count were observed and the F ratio of the phagocytic index was also significant. Regressedvalues revealed maximum linearity in the case of the phagocytic index, moderate linearity in thetotal WBC count and lowest linearity in the antibody response.
Cite this article:
Sanjiv Kumar Biradar, Chandra Kishore Tyagi. Phytochemical Screening and Immunomodulatory Activities of Methanolic Extract of Eclipta alba and Centella asiatica. Res. J. Pharmacology and Pharmacodynamics.2021; 13(1):5-8. doi: 10.5958/2321-5836.2021.00002.1
Cite(Electronic):
Sanjiv Kumar Biradar, Chandra Kishore Tyagi. Phytochemical Screening and Immunomodulatory Activities of Methanolic Extract of Eclipta alba and Centella asiatica. Res. J. Pharmacology and Pharmacodynamics.2021; 13(1):5-8. doi: 10.5958/2321-5836.2021.00002.1 Available on: https://rjppd.org/AbstractView.aspx?PID=2021-13-1-2
REFERENCES:
1. Atal CK, Sharma ML, Khariya A (1986) Immunomodulating agents of plant origin. J Ethnopharmacol 18: 133-141.
2. Boiteau P, Bazas A, Lederer E, Polonsky J (1949) Derivatives of Centella asiatica used against leprosy. Nature 163: 258.
3. Chatterjee RK, Fatma N, Jain RK, Gupta CM, Anand M (1998) Litomoidescarinii in rodents. Immunomodulation in potentiating action of diethylcarbamazine. J Pn J Exp Med 58: 243-248.
4. Chopra RN, Nair SL, Chopra IC (1958) In: Glossary of Indian medicinal plants: 353. CSIR, New Delhi Chopra RN, Nair SL, Chopra IC (1966) In: Glossary of Indian Medicinal Plants: 104. CSIR, New Delhi.
5. DiCarlo J, Haynes LJ, Sliver NJ, Phillips GH (1964) Reticuloendothelial system stimulants of botanical origin. J Reticuloendothelial Soc 1: 224-232.
6. Dua PR, Shankar G, Srimal RC, Saxena KC, Saxena RP, Puri A, Dhawan BN (1989) Adaptogenic activity of Indian Panaxpseudoginseng. Ind J Exp Biol 27: 631-634.
7. Hartwell JL (1969) Plants used against cancer. A survey. Lloydia 32: 153-205.
8. Inamdar PK, Yeole RD, Srivastava, de Souza NJ (1996) Stability study of the active constituents in the Centella asiatica extract formulations. Drug Dev. Industr Pharm 22: 211-216.
9. Ingolfsdottir K, Jurcic K, Fischer B, Wagner H (1994) Immunomodulatory active polysaccharide from Cetrariaislandica. Planta Med 60: 527-531.
10. Kartnig T (1988) In: Herbs, spices and medicinal plants- resent advances in Botany, Horticulture and Pharmacology: 145-173. 3rd edn.,
11. Oryx press, Phoenix Labadie RP, van der Nat JM, Simons JM, Kroes BH, Kosai S, van den Berg AJ, Hart LA, van der Sluis WG, AbeysekeraA, Bamunnarachi A, de Silva KT (1989) An ethnopharmacognostic approach to the search for immunomodulators ofplant origin. Planta Med 55: 339-348.
12. Leal LK, Ferriera AA, Bezerra GA, Matos FJ, Viana GS (2000) Antinociceptive, antinflammatory and bronchodialatoractivities of Brazilan medicinal plants containing Coumarin; a comparitive study. J Ethnopharmacol 2:51-159.
13. Lee GI, Young HA, Rakimn J, Nakagawa H, Tsurufuji S, Chang IM, Kim Y (1995) Inhibitory effects of orientalherbal medicines on IL-8 induction lipopolysaccharide activatedrat macrophages. Planta Med 65: 26-30.
14. Nagarkatti KM, Rege NN, Desai NK, Dahanukar SA (1994) Modulation of kuffer cell activity by Tinospora cordifolia in liver damage. J Postgrad Med 40: 65-67.
15. Patil JS, Nagavi BG, Ramesh M, Vijayakumar GS (1998) Astudy on the Immunostimulant activity of Centella asiatica in rats. Ind Drugs 38: 711-714
16. Puri A, Saxena RP, Saxena KC, Srivastava V, Tandon JS (1993) Immunostimulating agents from Andrographis paniculata. J Nat Prod 56: 995-999.
17. Shukla A, Rasik AM, Dhavan BN (1999a) Asiaticoside- inducedelevation of anti-oxidant levels in healing wounds. Phytother Res 13: 50-54.
18. Shukla A, Rasik AM, Jain GK, Shankar R, Kulshrestha DK, Dhavan BN (1999b) In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J Ethnopharmacol 65: 1-11
19. Upadhyay RK, Pandey MB, Jha RN, Pandey VB (2001) Eclalbatin, a triterpenesaponin from E. alba. Asian Nat Prod Res 3: 213-217.
20. Veerendrakumar, Gupta YK (2002) Effect of different extractson cognition and markers of oxidative stress in rats. J Ethnopharmacol 2: 253-260.
21. Wagner H, Jurcic K (1991) Assays for immunomodulation and effects on mediators of inflammation. In: Methodsin plant Biochemistry: 207, Vol. 6, Academic press, London
22. Wong SM, Antus S, Gottsegen A, Fessler B, Rao GC, SonnenbichlerJ, Wagner H (1988) Wedelolactone andcoumestan derivatives as new antihepatotoxic and antiphlogistic principles. Arzneim-Forsch/ Drug-Res 38:661-665.
23. Zafar R, Sagar BPS (1999) In vitro plant regeneration of Eclipta alba and increased production of coumestans. Fitoterapia 70: 348-356.