Venkateswaramurthy N, Ashli Raj V, Nisharani SS, Limna AL, Chandini S, Sambathkumar R
Venkateswaramurthy N*, Ashli Raj V, Nisharani SS, Limna AL, Chandini S, Sambathkumar R
Department of Pharmacy Practice, J.K.K. Nattraja College of Pharmacy, Kumarapalayam - 638183, India.
Volume - 13,
Issue - 3,
Year - 2021
Wound infection has always been a major complication of surgery and trauma. The aim of our study was to determine the bacteriology of wound infections and to study the antibiotic susceptibility pattern of the isolates. The study was conducted in tertiary care hospital, Erode. The design of the study was a prospective type. Pus swabs / specimens were collected from hospitalized patients who developed wound infections. Bacterial pathogens were identified by conventional biochemical methods according to standard microbiological techniques. Antimicrobial susceptibility was performed on Muller – Hinton agar by the standard disk diffusion method. The incidence of wound infection was more common in males (63%) than in females (37%). Out of 100 culture positive samples, 61 samples were collected from diabetic ulcer patients, 20 samples were from ulcer patients, and 19 samples were from post operative patients. Of the 100 samples (culture positive), 71% collected samples showed mono-microbial growth, 29% showed two type of microbial growth. The prevalence of S. aureus (62.87%) from different wound infections was found to be high, followed by E.coli (48.65%), Klebsiella (30.21%), Pseudomonas (22.16%). Organisms showed diversity in the sensitivity pattern towards the antibiotics tested. High level of sensitivity was observed to Imipenem, Piperacillin tazobactum and Amikacin. High level of resistance was observed to Cephalosporins and Penicillin derivatives. We concluded that the sensitivity pattern of the antibiotics is not based on the infection site but on the type of organisms. This study gives us an insight to the current state of causative pathogens and their sensitivity to different antibiotics used in tertiary care hospital, Erode. The data of this study may be used to determine trends in antimicrobial susceptibilities and to modify antibiotic policy of the Hospital.
Cite this article:
Venkateswaramurthy N, Ashli Raj V, Nisharani SS, Limna AL, Chandini S, Sambathkumar R. Bacteriology of wound infections and Antibiotic susceptibility pattern of the Isolates. Research Journal of Pharmacology and Pharmacodynamics. 2021; 13(3):81-5. doi: 10.52711/2321-5836.2021.00017
Venkateswaramurthy N, Ashli Raj V, Nisharani SS, Limna AL, Chandini S, Sambathkumar R. Bacteriology of wound infections and Antibiotic susceptibility pattern of the Isolates. Research Journal of Pharmacology and Pharmacodynamics. 2021; 13(3):81-5. doi: 10.52711/2321-5836.2021.00017 Available on: https://rjppd.org/AbstractView.aspx?PID=2021-13-3-1
1. Uwaezuoke JC, Nnodim JK. Bacteriology of Different Wound Infections and Their Antimicrobial Susceptibility Patterns in Owerri. Journal of Progressive Research in Biology. 2015; 1: 67- 69.
2. Mohammed, Adeshina G, Ibrahim Y. Retrospective incidence of wound infections and antibiotic sensitivity pattern: a study conducted at the Aminu Kano Teaching Hospital, Kano, Nigeria. International Journal of Medicine and Medical Sciences. 2013; 5: 60 -66.
3. Girish MB, Kumar TN. Culture and sensitivity pattern of microorganism isolated from diabetic foot infections in a tertiary care hospital. Journal of Biomedical and Pharmaceutical Research. 2011; 1: 34 – 40.
4. Walter CJ, Dumville JC, Sharp CA, Page T. Systematic review and meta-analysis of wound dressings in the prevention of surgical-site infections in surgical wounds healing by primary intention. British Journal of Surgery. 2012; 99: 1185-94.
5. Anderson DJ, Sexton DJ. Epidemiology and pathogenesis of and risk factors for surgical site infection. Up To Date. 2020. http://www. uptodate.com.
6. Mangram AJ, Horan TC, Pearson ML, Silver LC, Jarvis WR. Guideline for prevention of surgical site infection: 1999. Infection Control and Hospital Epidemiology. 1999; 27: 97-134.
7. Klevens RM, Edwards JR, Richards CL. Estimating health care associated infections and deaths in U.S hospitals. Public Health Research. 2007; 122: 160-66.
8. Cruse PJ, Foord R. The epidemiology of wound infection. Surgical Clinics of North America. 1980; 60: 27-40.
9. Rosenthal VD, Richtmann R, Singh S. Surgical site infections, International nosocomial infection control consortium report. Infection Control and Hospital Epidemiology. 2013; 34: 597-604.
10. Young MJ, Veves A, Boulton AJM. The diabetic foot: etiopathogenesis and management. Diabetes/Metabolism Research and Reviews. 1993; 9: 109-127.
11. Arulmoli SK, Sivachandiran S, Perera BJC. Prescribing patterns of antibiotics for children before admission to a paediatric ward in Jaffna Teaching Hospital. Sri Lanka Journal of Child Health.2009; 38: 121- 123.
12. Owens CD, Stoessel K. Surgical site infections: epidemiology, microbiology and prevention. Journal of Hospital Infection. 2008; 70: 3-10.
13. Bhatt CP, Lakhey M. The distribution of pathogen causing wound infection and their antibiotic susceptibility pattern. Journal of Nepal Health Research Council. 2007; 5: 22-26.
14. Clinical and Laboratory Standard Institute (CLSI): Performance standards for antimicrobial susceptibility testing twentieth informational supplement. 2010; 60–73.
15. Ohalete CN, Obi RK, Emea MC. Bacteriology of different wound infection and their antimicrobial susceptibility patterns in Imo state Nigeria. World Journal of Pharmaceutical Research. 2012; 1: 1155–1172.
16. Amlsha K, Adane M, Tamrat A, Tebkew A. Isolation and antimicrobial susceptibility pattern of Staphylococcus aureusin patients with surgical site infection at DebreMarkos Referral Hospital, Amhara Region, Ethiopia. Archives of Public Health. 2014; 72: 1-7.
17. Anne S, Bonnie LH, David GA, Benjamin AL. Microbiology of diabetic foot infections: from Louis Pasteur to crime scene investigation. BMC Medicine. 2015; 13: 2-13.
18. Prakash M, Lakshmi K, Anuradha S, Swathi GN. Bacteriological profile ad their antibiotic susceptibility pattern of cases of chronic suppurative otitis media. Asian Journal of Pharmaceutical and Clinical Research. 2013; 6: 210-212.
19. Psuedomonas aeruginosa in healthcare settings. www.cdc.gov/HAI/organisms/pseudomonas/pseudomonas.html. Updated on 7th may 2014. Assessed on 27th may 2020.
20. Bhavani G, Gopinath P. Detection of Biofilm among Clinical isolates of Acinetobacter baumannii by Tissue Culture Plate Method (TCP). Research Journal of Pharmacy and Technology. 2016; 9: 1635-1637.
21. Fysal Yousuf MA, Gopinath P. Detection of Slime among Clinical Isolates of Staphylococcus aureus and Pseudomonas aeruginosa. Research Journal of Pharmacy and Technology. 2016; 9: 2094-2096.
22. Abid M, Adeshina GO, Ibrahim YKE. Retrospective incidence of wound infections and antibiotic sensitivity pattern: A study conducted at the Aminu Kano Teaching Hospital, Kano, Nigeria. International Journal of Medical Sciences. 2013; 5: 60-66.
23. Shankar S, Arvind MV, Aakanksha P, Jovita S. Bacteriology of the burn wound at the Bai Jerbai Wadia Hospital for children, Mumbai, India—a 13-year study, Part I-Bacteriological profile. Indian Journal of Plastic Surgery. 2009; 42: 213-218.
24. Kulchenko NG, Chibisov SM, Eremina IZ, Vekilyan MA, Syatkin SP, Skorik AS, Myandina GI, Shevkun NA, Neborak EV. Dynamic Changes of the Microflora Sensitivity to Antibiotics in Patients with Chronic Calculous yelonephritis. Research Journal of Pharmacy and Technology. 2017; 10: 683-686.
25. Nanda A, Dhamodharan S, Nayak BK, Antibiotic Resistance Pattern Exhibited by Esbl (Extended Spectrum β-Lactamases) in Multidrug Resistant Strains, Escherichia coli. Research Journal of Pharmacy and Technology. 2017; 10: 3705-3708
26. Nkang AO, Okonko IO, Mejeha OK, Adewale OG, Udeze AO, Fowotade A, E. et al., Assessment of antibiotics susceptibility profiles of some selected clinical isolates from laboratories in Nigeria. Journal of Microbiology and Antimicrobials. 2009;1: 19- 26.
27. Ananda Deeban KBS, Gopinath P. Detection of High-Level Aminoglycoside Resistance (HLAR) among clinical isolates of Enterococci. Research Journal of Pharmacy and Technology. 2017; 10: 4195-4197.
28. Asha KR, Vedha PJ, Lavanya R, Kaviya U, Merlin JA. Assessment of Antibiotic Sensitivity Patterns in A Primary Care Hospital. Research Journal of Pharmacy and Technology 2018; 11: 3411-3414.
29. Erisken HM, Chugulu S, Kondo S, Lingaas E. Surgical site infections at Kilimanjaro Christian Medical Center. The Journal of Hospital Infection. 2003; 55: 14-20.
30. Veena S, Keerthana P, Jayabalaji A, Yasasve M, Vishal LA. Antibiotic and Metal Resistance offered by Aeromonas species isolated from Edible Fishes in Chennai, Tamil Nadu: A Field Investigation. Research Journal of Pharmacy and Technology. 2020; 13: 5197-5201.
31. Kishnani K, Bhandari S, Rathore KS. A Briefing of a Global Crisis: Antibiotic Resistance. Asian Journal of Research in Pharmaceutical Sciences. 2020; 10: 264-272.
32. Hisham AA, Mona AS, Amira MG, Azza AF. Antimicrobial Resistance Patterns of Proteus mirabilis isolates from Urinary tract, burn wound and Diabetic foot Infections. Research Journal of Pharmacy and Technology. 2018; 11: 249-252.
33. Samir IB, Nageeb AH, Rand SA, Sabrina AG. Urinary Tract Infection and Antibiotic Resistance among Pregnant and Non-pregnant females in UAE. Research Journal of Pharmacy and Technology. 2021; 14:461-465.
34. Niveditha, Srikanth, Rathai R, Shivamurthy. A Retrospective Evaluation of Compliance in Various Surgical Departments with Respect to Surgical Antibiotic Prophylaxis in a Tertiary Care Hospital. Research Journal of Pharmacy and Technology. 2013; 6: 749-752.