Author(s): Archita Srivastava, Archna Kumari, Abhishek Kumar Tripathi, Sunil Kumar Singh, Vineet Srivastava

Email(s): architasrivastava2009@gmail.com

DOI: 10.52711/2321-5836.2024.00010   

Address: Archita Srivastava1*, Archna Kumari2, Abhishek Kumar Tripathi1, Sunil Kumar Singh1, Vineet Srivastava1
1Department of Pharmacology, United Institute of Pharmacy, Naini, Prayagraj 211010, U.P, India.
2Department of Pharmacology, United Institute of Pharmacy, Naini, Prayagraj 211010, U.P, India.
*Corresponding Author

Published In:   Volume - 16,      Issue - 1,     Year - 2024


ABSTRACT:
The current study is design to evaluate the hepatoprotective action of an aqueous extract derived from the rhizome of Alpinia galanga in male Wistar rats. This assessment is conducted using a modified animal model that simulates liver toxicity induced by alcohol and carbon tetrachloride (CCl4) exposure. 30Wistar rats were divided into five groups (n=6) as the procedure was designed to last up to eight weeks. All the animals were fed with alcohol in free access, having a concentrationof 4% (v/v) ethanol, Additionally. The CCl4 is administered orally, which means it is given through the mouth. This can be done using a gavage needle or other oral administration methods. The dose of CCl4 is calculated based on the body weight of the subjects, with a dosage of 0.2ml per kilogram of body weight in ratio of 1:1.A drug Alpinia galanga aqueous extract (AEAG) was dissolved in unionized water and administered orally using an oral gauge in a single dosage at a rate of 500mg/kg body weight. In addition, the standard group received a standard medication, silymarin, at a dose of 25 mg/kg. Rats were killed at the conclusion of the trial, and a liver were taken for histological investigations and other biochemical testing, including lipid profiles, liver function tests, antioxidant activity tests. Each experimental group's body weight was checked once each week. The results indicatethe curative group was only marginally different from the preventive group based on biochemical parameters, antioxidant activity, and histological evaluation, whereas the animal treated with the preventive group is much more similar to the standard treated group than the curative group.


Cite this article:
Archita Srivastava, Archna Kumari, Abhishek Kumar Tripathi, Sunil Kumar Singh, Vineet Srivastava. Assessment of Hepatoprotective Consequence of Alpinia galanga Extract against Ethanol and Carbon Tetrachloride Induced Liver Damage on Experimental Animal. Research Journal of Pharmacology and Pharmacodynamics. 2024;16(1):52-7. doi: 10.52711/2321-5836.2024.00010

Cite(Electronic):
Archita Srivastava, Archna Kumari, Abhishek Kumar Tripathi, Sunil Kumar Singh, Vineet Srivastava. Assessment of Hepatoprotective Consequence of Alpinia galanga Extract against Ethanol and Carbon Tetrachloride Induced Liver Damage on Experimental Animal. Research Journal of Pharmacology and Pharmacodynamics. 2024;16(1):52-7. doi: 10.52711/2321-5836.2024.00010   Available on: https://rjppd.org/AbstractView.aspx?PID=2024-16-1-10


REFERENCES:
1.    Zhao, Z. Sylvia, and Peter J. O'Brien. The prevention of CCl4-induced liver necrosis in mice by naturally occurring methylenedioxybenzenes. Toxicology and Applied Pharmacology. 1996; 140(2): 411-421.
2.    Bader, Ted, and Brent Korba. Simvastatin potentiates the anti-hepatitis B virus activity of FDA-approved nucleoside analogue inhibitors in vitro." Antiviral Research. 2010; 86(3): 241-245.
3.    Waring J.F., Jolly R.A., Ciurlionis R., Lum P.Y., Praestgaard J.T., Morfitt D.C., Buratto B., Roberts C., Schadt E., and Ulrich R.G. “Clustering of Hepatotoxins Based on Mechanism of Toxicity Using Gene Expression Profiles.” Toxicology and Applied Pharmacology. 2001; 175, 28-42.
4.    Rezvanfar, M. A., Sadrkhanlou, R. A., Ahmadi, A., and Shojaei-Saadi, H. A. Evaluation of hepatoprotective effect of aquatic extract of Alpinia galangaa on rats treated with alloxan. Toxicology Mechanisms and Methods. 2010: 20(60), 333-337.
5.    Mohan, H. (2010). Textbook of Pathophysiology Six Edition. Chapter 21, The Liver, Biliary Tract and Exocrine Pancreas. Page no. 617. New Delhi: Jaypee Brothers Medical Publication (P) Ltd.
6.    Waring, Jeffrey F., Robert A. Jolly, Rita Ciurlionis, Pek Yee Lum, Jens T. Praestgaard, David C. Morfitt, Bruno Buratto, Chris Roberts, Eric Schadt, and Roger G. Ulrich. "Clustering of hepatotoxins based on mechanism of toxicity using gene expression profiles. Toxicology and Applied Pharmacology. 2001; 175(1): 28-42.
7.    Chalasani, N., Bjornsson, E. Risk factors for idiosyncratic drug-induced ;liver injury. Gasteroenterology, 2010, 138(7); 2246-2259.
8.    Holt, M. P., and Ju, C. Mechanisms of drug-induced liver injury. The AAPS Jounal. 2006: 8(1), E48-E54.
9.    McGill, M. R., and Jaeschke, H. Metabolism and disposition of acetaminophen: recent advances in relation to hepatotoxicity and diagnosis. Pharmaceutical Research. 2013; 30(9), 2174-2187.
10.    World Health Organization (WHO). Global status report on alcohol and health 2012. Geneva: WHO.
11.    Whitney Jackson. Alcohol-Related Liver Disease. Reviewed/Revised May 2021 | Modified Sep 2022.
12.    World Health Organization (WHO). Global status report on alcohol and health 2018. Geneva: World Health Organization.
13.    Krishna V. Textbook of Pathology. Chapter, The Liver and Biliary System. Orient Longman Pvt. Ltd. 2004; page no. 790-812.
14.    Li F, Duan K, Wang C, McClain C and FengW Probiotics and Alcoholic Liver Disease: Treatment and Potential Mechanisms Gastroenterology Research and Practice. 2016, Article ID 5491465, 11 pages.
15.    Patel R, Mueller M. Alcoholic Liver Disease. [Updated 2022 Oct 24]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan.
16.    Lucey, M. P., Mathurin, P., and Morgan, T. R. Alcoholic hepatitis. New England Journal of Medicine. 2009; 360(26), 2758-2769.
17.    Kemelo, M. K., L. Wojnarova, N. Kutinová Canová, and H. Farghali. D-galactosamine/ lipopolysaccharide-induced hepatotoxicity downregulates sirtuin 1 in rat liver: role of sirtuin 1 modulation in hepatoprotection. Physiological Research. 2014; 63(5): 615.
18.    Lackner C, Spindelboek W, Hybaeck J, Douschan P, Rainer F, Terracciano L, Haas J, Berghold A, Bataller R, Stauber RE. Histological parameters and alcohol abstinence determine long-term prognosis in patients with alcoholic liver disease. J Hepatol. 2017; 66: 610-8.
19.    Nampoothiri SV, Prathapan A, Cherian OL, et al. Alpinia galanga (L.) Willd.: ethnopharmacology, phytochemistry, and pharmacology of an important traditional medicinal plant of South East Asia. Asian Pac J Trop Med. 2012; 5(10):763-70.
20.    Singh G, Kapoor IP, Pandey SK, et al. Chemistry and bioactivities of essential oils of some Ocimum species: an overview. Asian Pac J Trop Biomed. 2015; 5(5): 268-279.
21.    Boonyanugomol W, Chantaranothai P. A morphological identity crisis: lineage delimitation in the Thai species of Alpinia series Galanga (Zingiberaceae). PloS ONE. 2020; 15(7): e0236150.
22.    Kengkoom K, Suksamrarn A, Aroonluk J, et al. Alpinia galanga (Linn.) Willd. extracts reduce oxidative stress and inflammation in neuronal PC12 cells. J Med Assoc Thai. 2011; 94 Suppl 3:S77-85.
23.    Verma, Ramesh K., Mishra Garima, Singh Pradeep, K. K. Jha, and R. L. Khosa. Alpinia galanga-an important medicinal plant: a review. Der Pharmacia Sinica. 2011; 2(1): 142-154.
24.    Kwon HJ, Won YS, Park O, Chang B, Duryee MJ, Thiele GE, Matsumoto A, Singh S, Abdelmegeed MA, Song BJ. Aldehyde dehydrogenase 2 deficiency ameliorates alcoholic fatty liver but worsens liver inflammation and fibrosis in mice. Hepatology. 2014; 60: 146-157.
25.    Furuya S, Chappell GA, Iwata Y, Uehara T, Kato Y, Kono H, Bataller R. and Rusyn I. A Mouse Model Of Alcoholic Liver Fibrosis-Associated Acute Kidney Injury Identifies Key Molecular Pathways. Toxicol Appl Pharmacol. 2016; 310: 129-139.
26.    Chen, Z., Wu, A., Jin, H., and Liu, F. β-Sitosterol attenuates liver injury in a rat model of chronic alcohol intake. Archives of Pharmacal Research. 2020; 43(11): 1197-1206.
27.    Wang, X., Dong, K., Ma, Y., Jin, Q., Yin, S., and Wang, S. Hepatoprotective effects of chamazulene against alcohol-induced liver damage by alleviation of oxidative stress in rat models. Open Life Sciences. 2020; 15(1): 251-258.
28.    Atef, M. M., Hafez, Y. M., Alshenawy, H. A., and Emam, M. N. Ameliorative effects of autophagy inducer, simvastatin on alcohol‐induced liver disease in a rat model. Journal of Cellular Biochemistry. 2019; 120(5): 7679-7688.
29.    Luanna Melo Pereira Fernandes, Klaylton Sousa Lopes, LuanaNazaré Silva Santana, Enéas Andrade Fontes-Júnior, Carolina Heitmann Mares AzevedoRibeiro, Márcia Cristina Freitas Silva, Ricardo Sousa de Oliveira Paraense, Maria Elena Crespo-López, Antônio Rafael Quadros Gomes, Rafael Rodrigues Lima, Marta ChagasMonteiro and Cristiane Socorro Ferraz Maia (2018) Repeated Cycles of Binge-Like Ethanol Intake in Adolescent Female Rats Induce Motor Function Impairment and Oxidative Damage in Motor Cortex and Liver, but Not in Blood.” Hindawi Oxidative Medicine and Cellular Longevity. ID 3467531.
30.    Subir Kumar Das, D.M. Vasudevan. Alcohol-induced oxidative stress. Life Sciences. 2007; 81: 177–187. doi:10.1016/j.lfs.2007.05.005.
31.    Campos-Shimada, Lilian Brites, Eduardo Hideo Gilglioni, Rosângela Fernandes Garcia, ElismariRizato Martins-Maciel, Emy Luiza Ishii-Iwamoto, and Clairce Luzia Salgueiro-Pagadigorria. "Superoxide dismutase: a review and a modified protocol for activities measurements in rat livers. Archives of Physiology and Biochemistry. 2020; 126(4): 292-299.
32.    Bakala, Hilaire, Maud Hamelin, Jean Mary, Caroline Borot-Laloi, and Bertrand Friguet. Catalase, a target of glycation damage in rat liver mitochondria with aging. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2012; 1822(10): 1527-1534.
33.    Karsdal, Morten A., Samuel J. Daniels, Signe Holm Nielsen, Cecilie Bager, Daniel GK Rasmussen, Rohit Loomba, Rambabu Surabattula et al. Collagen biology and non‐invasive biomarkers of liver fibrosis." Liver International. 2020; 40(4): 736-750.
34.    Mahae, Nopparat, and Siree Chaiseri. Antioxidant activities and antioxidative components in extracts of Alpinia galanga (L.) Sw. Agriculture and Natural Resources. 2009;43(2): 358-369.
35.    Lissa Paul, Ramya K. R.  Knowledge and Attitude towards Alcoholism among Adolescents. Asian J. Nur. Edu. and Research. 2012; 2(4): 212-214
36.    Ramya K. R., Sr.Lisa Paul. Psychosocial problems of wives of alcoholics. Asian J. Nur. Edu. and Research. 2013; 3(1): 29-30
37.    Priyanga Rangasamy, Vadakkenchery Salimudheen Hansiya, Palanisamy Uma Maheswari, Thamburaj Suman, Natesan Geetha. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoriaternatea L. Blue Flowered Leaves. Asian J. Pharm. Ana. 2019; 9(2): 67-76.
38.    Pradeep Kumar Samal. Antioxidant activity of Strobilanthesasperrimus in albino rats. Asian J. Pharm. Res. 2013; 3(2): 71-74.
39.    P. Muthukumaran, S. Salomi, R. Umamaheshwari. In vitro Antioxidant Activity of Premnaserratifolia Linn. Asian J. Res. Pharm. Sci. 2013; 3(1): 15-18.
40.    Pradeep Kumar Samal. Hepatoprotective activity of Ardisia solanacea in CCl4 induced Hepatoxic albino rats. Asian J. Res. Pharm. Sci. 2013; 3(2) : 79-82.
41.    Yogita. Chowdhary. Development and Evaluation of Dashmula plants of Ayurveda. Asian J. Pharm. Tech. 2018; 8(1):08-12.
42.    Ramya K. R. , Sr.Lisa Paul. Psychosocial problems of wives of alcoholics. Asian J. Nur. Edu. and Research. 2013;  3(1): 29-30
43.    Daniel Silas Samuel, R.V.Geetha. Antioxidant Activity of Dry Fruits: A Short Review. Research J. Pharm. and Tech. 2014; 7(11): 1319-1322.

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