Author(s):
B. D. Prasad, Ajey B. Gadagi, S. Dadakhalandar, Dyamanagouda, Jameer, Vikas, Nagaraj, Veeresh, Sharavan Naragund
Email(s):
ajeygadagi@gmail.com
DOI:
10.52711/2321-5836.2026.00015 
Address:
B. D. Prasad1, Ajey B. Gadagi2, S. Dadakhalandar3, Dyamanagouda3, Jameer4, Vikas4, Nagaraj4, Veeresh4, Sharavan Naragund5
1HOD and Professor, Dr. Gurachar Nargund College of Pharmacy, Muradi, Koppal.
2Associate Professor, Dr. Gurachar Nargund College of Pharmacy, Muradi, Koppal.
3Assistant Professor, Dr. Gurachar Nargund College of Pharmacy, Muradi, Koppal.
4Student, Dr. Gurachar Nargund College of Pharmacy, Muradi, Koppal.
5Principal, Dr. Gurachar Nargund College of Pharmacy, Muradi, Koppal.
*Corresponding Author
Published In:
Volume - 18,
Issue - 1,
Year - 2026
ABSTRACT:
The present research was undertaken to evaluate the in-vitro anticoagulant activity of aqueous and ethyl acetate leaf extracts of Syzygium cumini. The plant leaves were collected, shade-dried, powdered, and subjected to extraction using distilled water and ethyl acetate. The obtained extracts were screened for phytochemical constituents, which revealed the presence of carbohydrates, tannins, flavonoids, and glycosides, indicating the potential biological activity of the plant. The anticoagulant activity was determined by Prothrombin Time (PT) method using platelet-poor plasma isolated from healthy human volunteers. Plasma samples were treated with different concentrations of plant extracts ranging from 0.125 g/ml to 1 g/ml, and the clotting time was observed after the addition of calcium chloride. The findings demonstrated that both aqueous and ethyl acetate extracts significantly prolonged the prothrombin time in a concentration-dependent manner. The aqueous extract showed the highest anticoagulant activity, increasing the clotting time up to 48 minutes and 02 seconds at a concentration of 1 g/ml, whereas the ethyl acetate extract extended the clotting time to 45 minutes and 57 seconds at the same concentration. These results were notably higher compared to the normal control clotting time of 8 minutes and 16 seconds. The enhanced anticoagulant potential may be attributed to phenolic compounds and flavonoids that are known to inhibit clotting factor activation or chelate calcium ions essential for blood coagulation. Result: The normal plasma exhibited clot formation within 8 minutes and 16 seconds, indicating a normal physiological response. When plasma was treated with the standard anticoagulant (EDTA), the clotting time increased to 23 minutes and 14 seconds, confirming test accuracy. Upon treatment with plant extracts, the clotting time increased progressively with concentration. The aqueous extract produced the highest clotting time of 48 minutes and 02 seconds at 1 g/ml, while the ethyl-acetate extract prolonged clotting to 45 minutes and 57 seconds. This indicates a strong dose-dependent anticoagulant effect, with aqueous extract showing greater potency due to higher solubility and concentration of phenolic compounds in water.
Cite this article:
B. D. Prasad, Ajey B. Gadagi, S. Dadakhalandar, Dyamanagouda, Jameer, Vikas, Nagaraj, Veeresh, Sharavan Naragund. In-Vitro Anti-Coagulant Activity of Ethyl Acetate and Aqueous Extract of Syzygium cumini Leaves on Normal Blood Plasma. Research Journal of Pharmacology and Pharmacodynamics. 2026;18(1):111-4. doi: 10.52711/2321-5836.2026.00015
Cite(Electronic):
B. D. Prasad, Ajey B. Gadagi, S. Dadakhalandar, Dyamanagouda, Jameer, Vikas, Nagaraj, Veeresh, Sharavan Naragund. In-Vitro Anti-Coagulant Activity of Ethyl Acetate and Aqueous Extract of Syzygium cumini Leaves on Normal Blood Plasma. Research Journal of Pharmacology and Pharmacodynamics. 2026;18(1):111-4. doi: 10.52711/2321-5836.2026.00015 Available on: https://rjppd.org/AbstractView.aspx?PID=2026-18-1-15
REFERENCE:
1. S. Palta, R. Saroa, A. Palta. Overview of the coagulation system. Indian Journal of Anaesthesia. 2022; 66(3): 242–250.
2. F.R. Rosendaal. Causes of venous thrombosis: A multicausal perspective. Thrombosis Journal. 2019; 17(1):2–9.
3. J. Riedl, C. Ay. Venous thromboembolism: Risk factors, prevention, and management. Blood Reviews. 2023;60:101073.
4. J.A. Heit. Epidemiology of venous thromboembolism. Nature Reviews Cardiology. 2015;12(8):464–474.
5. A. Srivastava, et al. WFH Guidelines for the Management of Hemophilia, 4th edition. Haemophilia. 2023;29(Suppl. 1):1–158.
6. G. Denas, et al. Emerging anticoagulants: Focus on direct oral anticoagulants. Thrombosis Research. 2011; 127(Suppl. 3):S113–S118.
7. Q. Lai, et al. Anticoagulation therapy prevents portal-splenic vein thrombosis after splenectomy with gastroesophageal devascularization. World Journal of Gastroenterology. 2011;17(28):3298–3303.
8. E. Biffin, E.J. Lucas, L.A. Craven, I. Ribeiro da Costa, M.G. Harrington, M.D. Crisp. Evolution of exceptional species richness among lineages of fleshy-fruited Myrtaceae. Annals of Botany. 2010;106(1):79–93.
9. M. Ayyanar, P. Subash-Babu. Syzygium cumini (L.) Skeels: A review of its phytochemical constituents and traditional uses. Asian Pacific Journal of Tropical Biomedicine. 2011; 1(4): 240–246.
10. Z.P. Ruan, L.L. Zhang, Y.M. Lin. Evaluation of the antioxidant activity of Syzygium cumini leaves. Molecules. 2008; 13(10): 2545–2556.
11. B.G. Lima, B.P. Silva, L.C. Santos, V.L. Almeida. Correlation of anti-inflammatory activity with phenolic content in the leaves of Syzygium cumini (L.) Skeels (Myrtaceae). Revista Brasileira de Farmacognosia. 2007;17(3): 290–295.
12. A.C. Oliveira, et al. Antimicrobial activity of Syzygium cumini (Myrtaceae) leaves extract. Brazilian Journal of Microbiology. 2007; 38(2): 381–384.
13. S. Srivastava, R. Kapoor, A. Thathola, R.P. Srivastava. Pharmacological potentials of Syzygium cumini: A review. Journal of Complementary and Integrative Medicine. 2013; 10(1): 1–13.
14. D. Ezhilarasan, T. Lakshmi, M. Subha. Syzygium cumini extract induces reactive oxygen species–mediated apoptosis in human oral squamous carcinoma cells. Journal of Oral Pathology&Medicine. 2018;47(9): 872–879.
15. A.K. Prusty, T. Ghosh, S.K. Sahu. Anthelmintic, antimicrobial and antipyretic activity of various extracts of Clerodendrum infortunatum Linn. leaves. Oriental Pharmacy and Experimental Medicine. 2008; 8(4): 374–379.
16. R. Ahmed, M. Tariq, M. Hussain, A. Andleeb, M.S. Masoud, I. Ali, F. Mraiche, A. Hasan. Phenolic contents-based assessment of therapeutic potential of Syzygium cumini leaves extract. PLOS ONE. 2019;14(8):e0221318. doi: 10.1371/journal.pone.0221318 available on https://doi.org/10.1371/journal.pone.0221318.
17. M. Hoffman, D.M. Monroe. Coagulation 2006: A modern view of hemostasis. Hematology/Oncology Clinics of North America. 2007; 21(1): 1–11.
18. G. Mallika, V. Tulasi Das. Impact of Stress on working performance of Nurses and Organizational effectiveness in Hospitals. Asian Journal of Management. 2020; 11(3): 225–232. doi: 10.5958/2321-5763.2020.00035.9 available on https://ajmjournal.com/AbstractView.aspx?PID=2020-11-3-1.